Part:BBa_K2050422
Mouse H1 promoter (RNA Polymerase III promoter)
RNA polymerase III is responsible for transcribing small RNA molecules including the 5S rRNA and tRNAs. RNA polymerase III promoters, including the H1 promoter, is useful in creating synthetic functional RNA-encoding genes. RNA polymerase III promoters are considerably smaller than RNA polymerase II promoters and initiates expression at ten times the rate RNA polymerase II promoters do. Examples on using functional RNA genes include miRNA expression, guide RNA expression for CRISPR technology, and immunostimulatory RNA (isRNA) expression, the latter used in many projects (including ours) on vaccination.
The use of RNA molecules as a vaccine adjuvant hinges on the interaction of those RNA molecules with the retinoic acid-inducible gene 1 (RIG-1) receptor, which is a pathogen-associated molecular pattern recognition receptor specialized in detecting non-self RNA molecules and triggering the innate immunity upon recognizing such molecules. The presence of 5'-PPP on these molecules distinguish them from self RNA molecules, in which the 5'-PPP is usually moved or masked. Because RNA polymerase III transcription does not initiate 5-capping like RNA polymerase II transcription does, the use of RNA polymerase III promoters have this as an additional advantage for isRNA expression. The use of a mouse H1 promoter instead of that of human is preferable for human applications as it limits the probability for homologous recombination with the human genome.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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